Journal: CNS & neurological disorders drug targets

Article Title: Age-dependent microglial activation in immature brains after hypoxia-ischemia

doi:

Figure Lengend Snippet: A–C. Representative flow cytometry scatter plots of CD11b+/CD45+ cells in different brain regions (hippocampus, cortex, and striatum). Data were collected from the contralateral (CL) and ipsilateral (IL) hemispheres in P9 mice at 2 days post-HI (A) or 9 days post-HI (B; C). Y-axis: number of CD11b+ cells. X-axis: number of CD45+ cells. Dashed red line in the upper right quadrants: CD11b+/CD45+ cell population gated for further analysis in Figure 2–3.

Article Snippet: Monoclonal rat anti-mouse cluster of differentiation molecule 11b (CD11b) antibody and rat anti-mouse CD45-FITC antibody were from AbD Serotec (Raleigh, NC).

Techniques: Flow Cytometry

Journal: CNS & neurological disorders drug targets

Article Title: Age-dependent microglial activation in immature brains after hypoxia-ischemia

doi:

Figure Lengend Snippet: The number of CD11b+/CD45+ microglia/macrophage in hippocampus, cortex, or striatum was analyzed at day 1–17 post-HI via flow cytometry. A–D: P9 brains. A′– D′: P30 brains. The relative change of microglia/macrophage in the three brain regions of the ipsilateral hemisphere was normalized with the contralateral hemispheres (D and D′). Brain samples were pooled for this analysis (five animals for P9 and four animals for P30). Values are expressed as mean (A–C, A′– C′) and mean ± S.D. (D, D′), n = 3–4. * = p < 0.05 vs corresponding P30.

Article Snippet: Monoclonal rat anti-mouse cluster of differentiation molecule 11b (CD11b) antibody and rat anti-mouse CD45-FITC antibody were from AbD Serotec (Raleigh, NC).

Techniques: Flow Cytometry

Journal: CNS & neurological disorders drug targets

Article Title: Age-dependent microglial activation in immature brains after hypoxia-ischemia

doi:

Figure Lengend Snippet: Representative FACS plots showing HI triggered selective elevation of CD45 expression in the CD11b+/CD45+ microglia from the ipsilateral hippocampus, cortex, and striatum in P9 mice. A–D: P9 brains. A′–D′: P30 brains. Data were collected at the peak level of the microglial activation in each brain region (day 2 post-HI for hippocampus, day 9 post-HI for cortex and striatum). Dotted red box: CD45 medium expression histograms. Expression of CD45 in the CD11b+ microglial cells in P9 brains shifted from a low (<102) to medium (102–103) or higher level (> 103) after HI (reflected in the right shifted, broadened histogram curve). The proportion of the CD11b+/CD45+ cell population with low, medium, and high CD45 expression in each brain region are shown for P9 (D) and P30 (D′) mice. Values are expressed as mean ± S.D. (n = 3). * = p < 0.05 vs corresponding contralateral. # = p < 0.05 vs. corresponding P9. E. E′. The proportion of the CD11b+/CD45+ cell population with low, medium, and high CD45 expression in each brain region from naïve P9 (E) and P30 (E′) mice.. ND: not detectable. Values are expressed as mean ± S.D., n = 3. * = p < 0.05 vs. corresponding low, # = p< 0.05 vs. either corresponding cortex or striatum.

Article Snippet: Monoclonal rat anti-mouse cluster of differentiation molecule 11b (CD11b) antibody and rat anti-mouse CD45-FITC antibody were from AbD Serotec (Raleigh, NC).

Techniques: Expressing, Activation Assay

Journal: American Journal of Translational Research

Article Title: Improvement of endometrial thickness and in vitro fertilization outcomes in patients with Asherman’s refractory endometrium using autologous mesenchymal stem cells from the stromal vascular fraction

doi: 10.62347/uagf1249

Figure Lengend Snippet: Figure 2. Characterization of cultured human adipose-derived mesenchymal stem cells (ADMSC) contained in the stromal vascular fraction of the adipose tissue. A. Representative flow cytometry results of MSC markers: CD44, CD45, CD73, and CD90. B. Representative images of ADMSC differentiated into chondrocytes: ADMSC cultured under normal (left) and micro mass conditions (right) stained with Alcian Blue.

Article Snippet: MSCs were stained with antibodies against human CD73, CD90, CD105, CD44, CD45, CD34 (Cat# ab9375, ab133582, ab254022, Abcam), and viability marker VivaFIX (Cat#1351112, BioRad).

Techniques: Cell Culture, Derivative Assay, Flow Cytometry, Staining

Journal: Science Advances

Article Title: Knockdown of deleterious miRNA in progenitor cell–derived small extracellular vesicles enhances tissue repair in myocardial infarction

doi: 10.1126/sciadv.abo4616

Figure Lengend Snippet: ( A ) Immunohistochemical staining of ischemic hearts to detect CD63 + total macrophages and iNOS + CD63 + M1-type macrophages (CD63 for red and iNOS for green staining). Scale bars, 100 μm. ( B ) Quantification of the total number of CD63 + macrophages. *** P < 0.001 and **** P < 0.0001. ( C ) Quantification of the ratio of iNOS − CD63 + cells (M2 type) to iNOS + CD63 + cells (M1 type) ( n = 8). ** P < 0.01 versus M2 macrophage proportion in the PBS group. ( D ) Blood circulating CD45 − CD90 + cell population (MSC) in total PBMCs from cardiac IR rats. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: Twenty million PBMCs were incubated with allophycocyanin (APC)–conjugated anti-rat CD45 antibody (Miltenyi Biotec, North Rhine-Westphalia, Germany) and fluorescein isothiocyanate (FITC)–conjugated anti-rat CD90 antibody (Abcam, Cambridge, UK).

Techniques: Immunohistochemical staining, Staining